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Comparison of a modified
peptone water transport medium with two commercially
available transport media
for the recovery of aerobes from swab specimens
J. O. Isibor* and P. U. Amadi
Department
of Medical Laboratory Science, Faculty of Basic Medical
Sciences, College of Medicine, Ambrose Alli University,
P.M.B. 14, Ekpoma, Edo State, Nigeria.
*Corresponding author. E-mail:
Joe_Isibor@yahoo.com.
Accepted 31
March, 2009 |
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A laboratory modified peptone water medium was evaluated
alongside Stuart and Amies media for their relative
suitability as transport media for aerobic bacteria isolated
from wound specimens obtained from Central Hospital, Benin
City, Nigeria. The survival rates of isolates from the three
media were assessed quantitatively for a period of seven
successive days, using the pour-plate method for making
viable counts. The percentage of isolation was in the
following decreasing order, Pseudomonas aeruginosa
(36.4%), Klebsiella aerogenes (27.3%), Escherichia
coli (22.7%), Staphylococcus aureus (18.2%) and
Proteus sp (13.6%). The survival rate of
aerobic bacterial isolates was enhanced in the modified
peptone water transported medium (77.3%) compared with that
obtained in Stuart medium (22.7%) and Amies medium 54.5%).
Statistical analysis using the students t-test at 90%
confidence limit showed significant difference (p < 0.10)
when modified peptone water was compared with both Stuart
and Amies transport media.
Key
words:
Modified peptone water, Stuart, Amies, transport medium,
comparison, swab specimens. |
