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Study of aqueous extract
of three medicinal plants on cell membrane–permeabilizing
and their surface properties
Gholamreza Dehghan Noudeh1*, Fariba Sharififar2,
Mehdi Khatib3, Effat Behravan4 and
Masoud Ahmadi Afzadi1
1Kerman
Pharmaceutics Research Centre, Kerman University of Medical
Sciences, Kerman, Iran.
2Department
of Pharmacogenozy, Faculty of Pharmacy, Kerman University of
Medical Sciences,
Kerman,
Iran.
3Department
of Pharmaceutics, Faculty of Pharmacy, Kerman University of
Medical Sciences, Kerman, Iran.
4Department
of Pharmacodynamy Toxicology, Faculty of Pharmacy, Mashhad
university of Medical Sciences, Mashhad, Iran.
*Corresponding author. E-mail:
grdehghan@yahoo.com.
Tel: +98-341-3205014. Fax: +98-341-3205215.
Accepted
2 November, 2009 |
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The aim of this study was to evaluate the effect of aqueous
extract of three medicinal plants, Artemisia dracunculus
L, Cuminum cyminum L and Heracleum
persicum Desf, which contain saponins on biological
membrane. Also in this study, some of their physicochemical
properties were studied. At the first step, the aqueous
extract of the plants were prepared, using maceration and
then the extracts were lyophilized. 0.2 ml of RBC was added
to 0.2 ml of different concentrations of each extract in
McIvan’s buffer, and then incubated in two different times
and temperatures. The absorbance of the samples was
determined by UV spectrophotometer. Among the three studied
extracts, A. dracunculus L showed the highest
hemolytic effect and the Heracleum persicum
Desf showed the lowest one. The values of emulsification
Index (E24) and foam formation activity (Fh)
showed for each extract the properties of surface activity.
Regarding the results of this study, when considering the
health of consumer, the use of aqueous extract of H.
persicum Desf, with low hemolytic effect is preferred
in pharmaceutical preparation. But if the hemolytic effect
were considered, the use of aqueous extract of A.
dracunculus L, with great hemolythc effect in comparison
to the two other extract, is preferred.
Key words:
Artemisia dracunculus L, Cuminum cyminum L,
Heracleum persicum Desf, biological membrane, hemolysis. |
