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In vitro
storage of synthetic seeds: Effect of different storage
conditions and intervals on their conversion ability
Muhammad Ikhlaq, Ishfaq Ahmad Hafiz1*,
Maurizio Micheli2, Touqeer Ahmad1,
Nadeem Akhtar Abbasi and Alvaro Standardi2
1Department
of Horticulture, Pir Mehr Ali Shah Arid Agriculture
University Rawalpindi, Pakistan.
2Department
of Agricultural and Environmental Sciences, Faculty of
Agriculture, University of Perugia, Italy.
*Corresponding author. E-mail:
decenthafiz@gmail.com.
Abbreviations: OMM,
Olive medium modified; RIM, root induction medium;
IBA, indole-3-butyric acid; MCTS, microcutting;
ABA, abscisic acid.
Accepted 29 July, 2010 |
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In vitro
derived shoots of olive cv. Moraiolo were employed in
synthetic seeds preparation by alginate encapsulation, and
then stored in artificial endosperm solution at cold (4°C)
and room storage (21 ± 2°C) conditions in interaction with
different storage intervals of 0, 15, 30, 45 and 60 days to
evaluate the comparative regrowth and conversion capacity of
synthetic seeds. Cold stored synthetic seeds were superior
in terms of their regrowth capacity than that of room stored
ones for all the growth parameters studied. A promising
degree of interaction was observed between 4°C and 45 days
of storage interval for regrowth percentage as well as for
shoot and root development. Moreover, an ascending trend
was recorded in conversion potential with an increase in
storage intervals up to 45 days (S3) whereas
there was a declining trend after that up to 60 days (S4).
Moreover plantlets regenerated from synthetic seeds, with 4
- 6 fully expanded leaves and well developed root system
were successfully acclimatized under ex vitro
conditions. The protocol can be used for germplasm exchange
of woody trees and preparation of synthetic seed.
Key word:
Synthetic, seed, olive, encapsulation, storage, conversion. |
