Full Length Research Paper

Verification of the utility of molecular markers linked to the multiple-allele male-sterile gene Ms in the breeding of male-sterile lines of Chinese cabbage (Brassica rapa)

Feng Hui*, Wang Lili, Wei Peng, Liu Zhiyong, Li Chengyu, Wang Yugang, Ji Ruiqin and Zhang Huaqing

Department of Horticulture, Shenyang Agricultural University 110161, China.

*Corresponding author. E-mail: peng8@163.com.  Tel: 86-13840065930.

Fax: 86-024-88487144.

Abbreviations: SCAR, Sequence-characterized amplified region; SSR, simple sequence repeat; MAS, marker-assisted selection; BSA, bulk segregant analysis.

Accepted 29 April, 2010

   Abstract

To verify the molecular markers linked to the genic multiple-allele male-sterile gene Ms, an F₁ plant, which was generated by crossing the inbred line a20 and the male-sterile plant of the genic multiple-allele male-sterile AB line, was backcrossed with an a20 plant to develop BC₄ and BC₅ populations. Sequence-characterized amplified region (SCAR) marker syau_scr01 and simple sequence repeat (SSR) marker syau_m13, which were linked to Ms, exhibited polymorphism between the 2 parents. The accuracies of these 2 markers in determining the plant genotype was 85 and 91.7%, respectively. The accuracy reached 100% when the 2 markers were used in combination. These results indicate that these 2 markers can be applied in the marker-assisted selection of the genic multiple-allele male-sterile line of Chinese cabbage.

Key words: Chinese cabbage, genic multiple-allele male sterility, marker-assisted selection, simple sequence repeat, sequence-characterized amplified region.