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Effect of
Sacoglottis gabonensis and Alstonia boonei on the
kinetics of Saccharomyces cerevisiae isolated from
palm wine
A. I. Elijah1*, P. C.
Ojimelukwe2, U. S. Ekong3 and N. U.
Asamudo4
1Department
of Food Science and Technology,
University of Uyo, PMB 1017, Uyo, Akwa Ibom State, Nigeria.
2Department
of Food Science and Technology, Michael Okpara University of
Agriculture, Umudike, PMB 7267, Umuahia, Abia State,
Nigeria.
3Department
of Pharmaceutics and Pharmaceutical Technology, Faculty of
Pharmacy, University of Uyo, PMB 1017, Uyo, Akwa Ibom State,
Nigeria.
4Department
of Microbiology, University of Uyo, PMB 1017, Uyo, Akwa Ibom
State, Nigeria.
*Corresponding author. E-mail:
aniplus_ai@yahoo.com. Tel: +2348037890522.
Abbreviations:
PW, Palm wine; SDA, Saboraud dextrose agar;
MYGP, malt extract yeast extract glucose peptone medium.
Accepted 2 August, 2010 |
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Sacoglottis gabonensis
and Alstonia boonei are botanicals used for the
preservation of palm wine in Southern Nigeria. This study
investigated the effect of S. gabonensis (0.625%) and A.
boonei (0.50%) on the kinetics of Saccharomyces
cerevisiae isolated from palm wine (PW). Concentrations
of the preservatives used in this study were optimal
concentrations of the preservatives that had preservative
effect on fermenting palm sap. The fermentation rate
constant, k, of 2.79 × 10-4 mol-1 s-1
obtained for untreated PW was higher than the k values for
PW treated with A. boonei (1.7 × 10-4 mol-1
s-1) and S. gabonensis (1.1 × 10-4
mol-1 sec-1). Both preservatives
enhanced yeast growth. The specific growth rates (µmax)
for the yeast were 0.43, 0.76 and 0.88 for the control,
samples treated with A. boonei and S. gabonensis,
respectively. However, the sedimentation rate of the yeast
was reduced by both preservatives, but A. boonei
produced the greatest effect. The utilization of these
botanicals for industrial fermentations involving yeast is
promising.
Key words:
Palm wine, preservation, Saccharomyces cerevisiae,
fermentation kinetic, growth kinetic, sedimentation rate,
Sacoglottis gabonensis, Alstonia boonei. |
