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Evaluation of
substrates from renewable-resources in biosurfactants
production by Pseudomonas strains
Sidnei Cerqueira
dos Santos1*, Luzimar Gonzaga Fernandez2,
Juan Carlos Rossi-Alva3 and Milton Ricardo de
Abreu Roque4
1Programa
de Pós-Graduação em Biotecnologia,
Departamento de
Ciências Biológicas, Universidade
Estadual de Feira de Santana, Bahia, Brazil.
2Laboratório
de Bioquímica, Biotecnologia e Bioenergia, Departamento de
Biofunção, Universidade Federal da Bahia, Bahia, Brazil.
3Laboratório
de Estudos em Meio Ambiente,
Superintendência de
Pesquisa e Pós-Graduação,
Universidade Católica do Salvador, Bahia, Brazil.
4Laboratório
de Biotecnologia e Ecologia de Microrganismos,
Departamento de Biointeração,
Universidade Federal da Bahia, Bahia, Brazil.
*Corresponding
author. E-mail:
sidneibio@yahoo.com.br. Tel: +557132838937.
Fax: +557132352416.
Abbreviations:
TSA, Triptic soyer agar;
MSM,
mineral saline medium;
TSB,
tryptic soy broth;
ST,
surface tension; CMC, critical micellar
concentration; SDS, sodium dodecyl sulfate; PAHs,
polycyclic aromatic hydrocarbons; LCFAs, long chain
fatty acids.
Accepted 16 July, 2010 |
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The aim of the present study is to evaluate biosurfactant
production by two strains of Pseudomonas spp. using
alternative carbon sources. The bacterial strains used were
isolated from the Municipal Park of Mucugê, Bahia State,
Brazil. Different vegetable oils and glycerol concentrations
were evaluated and used individually, as the sole source of
carbon and energy for the production of biosurfactant which
was measured by the test of emulsification and surface
tension. The best concentration of alternative substrates
achieved was 0.5%, with emulsion formation rates ranging
from 50 to 59% for both bacterial strains analyzed. The
free-cell broth of Pseudomonas strains had the
ability to reduce the surface tension of all culture media
tested, although it was more effective when glycerol was
used. The results confirm the potential of these alternative
substrates, based on the availability and low cost of these
raw materials, to
reduce the final production cost and also the expenses with
waste treatment.
Key words:
Pseudomonas,
biosurfactants, substrates, renewable-resources. |
