identification and cadmium adsorption of a high
cadmium-resistant Paecilomyces lilacinus
Xiaoxi Zeng1,2*, Jianxin Tang2,
Huaqun Yin1, Xueduan Liu1, Pei Jiang2
and Hongwei Liu1
of Minerals Processing and Bioengineering, Central South
University, Changsha, 410083, P.R.China.
Key Lab of Green Packaging and Biological Nanotechnology,
Hunan University of Technology, Zhuzhou, 412007, P. R.China.
email@example.com. Tel: +86 -731-26241803.
ITS, Internal transcribed spacers; SEM,
scanning electron microscopy; EDX, energy dispersive
X-ray; ROS, reactive oxygen species; PDA,
potato dextrose agar; PCR, polymerase chain reaction;
minimum inhibitory concentration; BLAST,
basic local alignment search tool; PHB,
poly-3-hydroxybutyrate; PCL, polycaprolactone.
Accepted 23 March, 2010
important roles in bioremediation. The present study was
carried out to investigate metal resistance and adsorption
strains. A cadmium-resistant fungus M1 was isolated from
Zhuzhou smelter in China. The 18S rRNA, internal transcribed
spacers (ITS) region and
gene of the strain were sequenced and aligned with the high
similar sequences published in GenBank. The morphology was
analyzed under scanning electron microscopy (SEM). The
growth in the 100 mg/l cadmium medium was investigated and
the biomass was analyzed by energy dispersive X-ray (EDX)
spectroscopy. The results showed that strain M1 which
tolerated 80 mM cadmium belong to Paecilomyces lilacinus.
Except for cadmium, the fungus showed resistance to zinc,
manganese, copper, lead, and cobalt. The cadmium bisorption
capacity of the fungus reached 24.23 mg/g during growth. EDX
confirmed that cadmium biosorption occurred on the fungus
biomass. The work suggested that P. lilacinus M1 was
a potential strain on cadmium bioremediation.
Paecilomyces lilacinus, cadmium-resistant,