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Antimicrobial activity of
photo-activated cow urine against certain pathogenic
bacterial strains
Ravi Kant Upadhyay*, Pratibha Dwivedi and Shoeb Ahmad
Department of Zoology, Deen Dayal Upadhyay (D D U) Gorakhpur
University, Gorakhpur, U.P. India.
*Corresponding author. E-mail:
rkupad@yahoo.com.
Accepted 27 November, 2009 |
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In the
present investigation, binary combination of photo activated
cow urine was determined against seven bacterial strains.
Photoactivated cow urine showed MIC value of 0.25
ml/ml
against Staphylococcus aureus (ATCC 25923),
Bacillus cereus (ATCC 11778), Lactobacillus
acidophilus (ATCC 53103 and Micrococcus luteus (ATCC
9341), while it had 0.125
ml/ml
against Escherichia coli (ATCC 25922). Binary
combinations of cow urine with Neem and Bavchi oil showed
synergistic effect as the MIC value obtained was between
0.125 - 0.25
ml/ml.
Similarly, photoactivated cow urine showed least MBC value
of 0.25 - 1.0
ml/ml.
Furthermore, growth inhibition zone diameters obtained in
the presence of photoactivated cow urine and its binary
combinations were found much larger than antibiotic drugs.
Neem oil showed highest inhibition zone diameter of 45 mm
against Streptococcus pneumoniae. Neem oil and cow
urine showed 33 - 35 mm inhibition zone diameter against
B. cereus, L. acidophilus, Micrococcus luteus, Klebsiella
pneumoniae and S. pneumoniae, while Bawchi oil and cow
urine combination showed a higher inhibition zone diameter
of 41 mm against K. pneumoniae. Photoactivated cow
urine showed 32 - 36 mm inhibition zone diameter
homogeneously against all bacterial strains. It proved very
high antimicrobial susceptibility of cow urine and essential
oils.
Key
words:
Antimicrobial activity, cow urine and essential oil. |
