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In vivo
extracellular matrix protein expression by human
periodontal ligament after stimulation with orthodontic
force
José A. Morales-González1*,
José Gutiérrez-Salinas2, Eduardo
Madrigal-Santillán1, Jaime Esquivel-Soto3,
Cesar Esquivel-Chirino3, Manuel García Luna y
González Rubio3 and Marco A. Becerril-Flores4
1Área
Académica de Farmacia, Instituto de Ciencias de la Salud,
Universidad Autónoma del Estado de Hidalgo, Mexico.
2Laboratorio
de Bioquímica y Medicina Experimental, División de
Investigación Biomédica, Centro Médico Nacional “20 de
Noviembre”, ISSSTE, México.
3Facultad
de Odontología, UNAM, Mexico.
4Área
Académica de Medicina, Instituto de Ciencias de la Salud,
Universidad Autónoma del Estado de Hidalgo, Mexico.
*Corresponding author. E-mail:
jmorales101@yahoo.com.mx.
Accepted 8
June, 2010 |
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It is well known that the orthodontic force applied to teeth
generates a series of events that remodel the periodontal
ligament (PDL). Extracellular matrix proteins (ECM) are
described as molecular regulators of these events. However,
the exact contribution of these proteins in human PDL
modeling by orthodontic force application in vivo is
not known. The aim of this present study was to evaluate the
protein expression of fibronectin, laminin and vitronectin
by human PDL from teeth on which orthodontic force was
applied. Twenty healthy individuals were included in the
study. PDL was obtained from teeth after a 3-week treatment
with orthodontic force. PDL-protein samples were separated
on 7.5% SDS-PAGE Western blot analysis with specific
monoclonal antibodies for fibronectin, laminin and
vitronectin. Bands were visualized with an enhanced
chemiluminescence detection system and densitometric.
Scanning of bands was carried out to compare differences in
protein expression. A significant increment in fibronectin
(13.9%), laminin (16.5%) and vitronectin (14.2%) expression
was found in PDL from teeth treated with orthodontic force
for 3 weeks in comparison with teeth in the control group.
Our results support the concept that molecular changes take
place by application of orthodontic forces to the PDL. Over
expression of these proteins suggests that extracellular
matrix (ECM) remodeling could be generated in response to
mechanical stress.
Key
words:
Extracellular matrix proteins, periodontal ligament,
orthodontic force. |
