|
The effect of valproic
acid on rat ovarium and the protective role of vitamin E and
folic acid: An ultrastructural study
Ayfer Aktas*, Yusuf Nergiz and Murat Akkuş
Departments
of Histology and Embryology, Faculty of Medicine, Dicle
University, Turkey.
*Corresponding author. E-mail: aaktas@dicle.edu.tr or
fuldic@gmail.com. Fax:
+90 412 2488440.
Abbreviations:
VPA, Valproic acid; mEH, microsomal epoxide
hydrolase; CIF, caspase inhibitory factor; ROS,
reactive oxygen species; PCOS, polycystic ovary
syndrome; AEDs, anti-epileptic drugs.
Accepted 2
August, 2010 |
|
This
study was undertaken to investigate the effect of valproic
acid and protective effects of vitamin E and folic acid on
rat ovary ultrastructural changes. Twenty-four Wistar rats
were used. Animals were divided into four groups. The first
group of rats was used as control. The second group was
injected valproic acid. The third group was injected
valproic acid + folic acid and the fourth group was given
valproic acid + vitamin E. At the end of the study, ovarium
tissues were taken under anesthesia. Tissues were prepared
and examined by transmission electron microscopy.
Microscopically, the groups are cited as follows:
Control
group
(in which the ovarium tissue was normal), valproic acid
group
(which
showed increase in lipid content plus mitochondrial
crystalysis seen in folliculer and theca interna cells of
rat ovarium),
valproic
acid + folic acid group (in which the theca interna and
granulosa cells of rat ovarium had normal appearance) and
valproic acid + vitamin E group (where all the organelles of
theca interna and granulosa cells of rat ovarium were
observed to be normal). Vitamin E and folic acid have
protective effects against valproic acid-induced tissue
damage in rat ovaries.
Key words:
Valproic acid,
vitamin E,
folic acid, ovarium, rat. |
