|
The isolation
and identification of predatory bacteria from a
Microcystis algal bloom
Jabulani R. Gumbo1,2*, Gina
Ross1 and Thomas E. Cloete1
1Department
of Microbiology and Plant Pathology, University of Pretoria,
Pretoria 0001, South Africa.
2Department
of Hydrology and Water Resources, University of Venda,
Thohoyandou, 0950, South Africa.
*Corresponding author. E-mail:
abulani_gumbo@yahoo.co.uk,
jabulani.gumbo@univen.ac.za.
Tel: +27 15 962 8563. Fax: +27 15 962 8597.
Accepted 13 August, 2009 |
|
Water samples were collected from a hypertrophic lake,
Hartbeespoort dam, where lytic or predatory bacteria were
isolated. The water samples were spread on BG 11 agar plates
where upon incubation, plaques were observed on
Microcystis lawns. Two isolates were further subjected
to screening to evaluate their lytic activities on
Microcystis cells and identification. Isolates B2 and
B16 had a lytic effect on the Microcystis cells with
isolate B16 having a greater effect than isolate B2.
Isolate B2 was identified as Pseudomonas stutzeri
with 99.9% certainty and B16 as Bacillus mycoides
with 99.7% certainty using the API system.
Key
words:
Microcystis, predator-prey ratio, Bacillus
mycoides, Pseudomonas stutzeri. |
