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Studies on bioemulsifier
production by Bacillus licheniformis PTCC 1595
Gholamreza Dehghan Noudeh1,2*, Mohammad Hasan
Moshafi1,2, Payam Kazaeli1,2 and
Farideh Akef3
1Department
of pharmaceutics, School of pharmacy, Kerman University of
Medical Sciences, Kerman, Iran.
2Pharmaceutics
Research Center, Kerman University of Medical Sciences,
Kerman, Iran.
3Pharmacist.
*Corresponding author. E-mail:
grdehghan@yahoo.com.
Tel:
++98-341-3220090-95. Fax: ++98-341-3220799.
Accepted 2
October, 2007 |
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Surfactants are amphipathic molecules which reduce surface
and interfacial tensions and widely used in pharmaceutical,
cosmetic, food and petroleum industries. Biosurfactants are
the structurally diverse group of surface-active molecules
synthesized by microorganisms. There are several advantages
for biosurfactants in contrast with chemical surfactants,
such as lower toxicity; higher biodegradability; better
environmental compatibility; higher foaming; high
selectivity and specific activity at extreme temperatures,
pH, and the ability to be synthesized from renewable
feed-stock. In the present study, the production of
bioemulsifer by Bacillus licheniformis PTCC 1595
was studied. B. licheniformis was grown in the
nutrient broth medium and bioemulsifer production was
evaluated every 24 h by surface tension and emulsification
index (E24). Then B. licheniformis PTCC 1595 was
grown in nutrient broth with different conditions in order
to get maximum production of bioemulsifer. The best culture
medium was found to be nutrient broth medium supplemented
with starch, Fe2+, Mn2+ and olive oil.
After growing the bacteria, the microbial biomass was
removed from the supernatant by acidic precipitation method.
Its amphipathic structure was established by biochemical and
spectroscopy methods and it was confirmed to be a
lipopeptide structure.
Key
words:
Bacillus licheniformis, bioemulsifer, surface
tension, emulsification index. |
