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Full Length Research Paper
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Phytochemicals and in
vitro antioxidant potentials of defatted methanolic
extract of Holarrhena floribunda leaves
Badmus, Jelili A.1*, Odunola, Oyeronke A.2,
Obuotor, Efere M.3 and Oyedapo, Oyeboade O.3
1Department
of Biochemistry, Ladoke Akintola University of Technology,
Ogbomoso, Nigeria.
2Department
of Biochemistry, University of Ibadan, Ibadan, Nigeria.
3Department
of Biochemistry, Obafemi Awolowo University, Ile Ife,
Nigeria.
*Corresponding author. E-mail:
jabadmus@gmail.com
or
jeli_b2002@yahoo.com. Tel: 234-8035850982.
Accepted 21
September, 2009 |
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Abstract |
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Plant-based dietary components and additives are known to
protect cells from deleterious effect of reactive oxygen
species (ROS). Proximate, phytochemical and antioxidant
potentials of methanolic extract of defatted Holarrhena
floribunda (G.Don) leaves were assessed using in
vitro systems such as, 1,1 diphenyl-2-picrylhydrazyl (DPPH)
radical,
hydroxyl radical,
nitric
oxide radical scavenging and inhibition of lipid
peroxidation. Total antioxidant activity was measured
using
phosphomolybdenum method. Total phenol content and the
reductive potential of the extract were also evaluated. The results of the proximate analysis revealed that the leaf contained
0.23% moisture, 12.8% ash, 9.62% crude fat, 23.3% crude
fibre, 21.17% protein and 32.68% carbohydrate while the
phytochemical constituents included alkaloid, saponin,
tannin
and cardiac glycosides. The leaf extract of H. floribunda (G.
Don) exhibited scavenging activity with IC50 of
12.63,
1,377.00
and 244.00 µg/ml for DPPH, hydroxyl radical and nitric oxide
radical respectively. The extract conferred 50% protection
at the concentration of 73.80 µg/ml on lipid peroxidation
induced by FeSO4 in egg yolk. Total antioxidant
capacity equivalent of gallic acid and vitamin C were 195.57
and 519.28 μg/mg of extract respectively and
total phenol content equivalent of gallic acid was
1427.87 µg/mg. The
reductive potential increased with increasing concentration
of extract.
The results
obtained from this study reveal that the extract is rich in
antioxidant components with several mechanisms of eliciting
antioxidant actions which provide scientific basis for its
use in folk medicine.
Key
words:
Reactive oxygen species, Holarrhena floribunda,
antioxidant, phytochemical. |
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