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Changes in
specific activity of ascorbate peroxidase during seed
development of pea (Pisum sativum L.) treated with
salicylic acid
Ghulam Murtaza1*, Rehana
Asghar2 and Syed Abdul Majid1
1Department of Botany,
University of Azad Jammu and Kashmir, Muzaffarabad,
Pakistan.
2Department of Botany, PMAS
Arid Agriculture University, Rawalpindi, Pakistan.
*Corresponding author. E-mail:
gmurtazaq@hotmail.com.
Abbreviations: SA,
Salicylic acid;
APX,
ascorbate peroxidase; STFS, seed treatment plus
foliar spray; ST, seed treatment; FS, foliar
spray;
EDTA, ethylenediaminetetraacetic
acid; BSA, bovine serum albumin;
ROS,
reactive oxygen species.
Accepted 16 July, 2010 |
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A field split-split plot experiment in randomized complete
block design was conducted during years 2003-04 and 2004-05
to evaluate the effects of salicylic acid (SA) at
concentrations of 0, 10-4 and 10-5 M
on four pea varieties (Meteor, Climax, Greenfeast and
Rondo). Four phenological growth stages were selected of
seed and fruit development (BBCH 73, BBCH 77, BBCH 83 and
BBCH 88) for enzyme assay. Aqueous SA was applied by three
different modes: Seed treatment, seed treatment plus foliar
spray and foliar spray.
Maximum ascorbate peroxidase (APX) activity was exhibited by
the variety Rondo as compared to other three varieties
during both years of study. At BBCH 77, all the varieties
showed maximum specific activities of APX which gradually
decreased in BBCH 83 and BBCH 88 phenological growth stages.
The specific activities of APX were recorded as maximum in
the pea seeds treated with SA concentration 10-4
M as compared to 10-5 M and 0 M during both years
of study. In comparison of modes of application of SA, it
was observed that maximum specific activity of APX was in
plants which were given seed treatment plus foliar spray (STFS)
as compared to only seed treatment (ST) or foliar spray
(FS).
Key words:
Salicylic acid, BBCH, pea, ascorbate peroxidase, foliar
application, Pisum sativum. |
