|
Molecular
characterization, polymorphism of growth differentiation
factor 5 gene and association with ultrasound measurement
traits in native Chinese cattle breeds
Yongfeng Liu1#, Yang Jiao1#,
Linsen Zan2*, Yaping Xin1, Linqiang Li1
and Wanqiang Tian1
1College
of Animal Science and Technology, Northwest A and F
University, Yangling, Shaanxi 712100, PR China.
2National
Beef cattle improvement centre of China,
Yangling, Shaanxi 7 12100, PR China.
*Corresponding
author. E-mail:
zanls@yahoo.com.cn. Tel: +86-29-87091923.
Fax: +86-29-87091148.
Abbreviations: GDF5,
Growth differentiation factor 5; MAS, marker-assisted
selection; SNP, single nucleotide polymorphism;
UMAR, ultrasound marbling score; UBF, ultrasound
backfat thickness; CDMP1, cartilage-derived
morphogenetic protein 1; TGF-b,
transforming growth factor-b; BMPs, bone
morphogenetic proteins; ULMA, ultrasound longissimus
muscle area; NCBI, national center for biotechnology
information; PCR, polymerase chain reaction; RFLP,
restriction fragment length polymorphism; CDS, coding
sequence.
#These Authors contributed equally to this paper.
Accepted 18 June, 2010 |
|
Growth differentiation factor 5 (GDF5), involved in
the development and maintenance of bone and cartilage, is an
important candidate gene for growth and carcass traits
selection through marker-assisted selection (MAS). Genomic
structural analysis showed that bovine GDF5 shares
much similarity with human GDF5. The latest findings
demonstrate that the single nucleotide polymorphism (SNP)
T586C in exon 1 is significantly associated with ultrasound
marbling score (UMAR) and ultrasound backfat thickness (UBF).
Furthermore, the analysis of T586C SNP marker shows there
are significant effects on the UBF (P = 0.0498) and on the
UMAR (P = 0.0058) in 465 individuals. These results clearly
suggest that the GDF5 gene is among target genes for
carcass traits in bovine reproduction and breeding.
Key words:
Cattle, GDF5 gene, ultrasound measurement, polymorphism,
association analysis. |
