|
Over-expression of Arabidopsis DnaJ (Hsp40) contributes to
NaCl-stress tolerance
Zhao Zhichang, Zhang Wanrong, Yan Jinping, Zhang Jianjun,
Liu Zhen Li Xufeng and Yi Yang
Key
Laboratory of Bio-resources and Eco-environment of Ministry
of Education, School of Life Science, Sichuan University,
Chengdu 610064, China.
*Corresponding author. E-mail:
zhaozhichang2001@163.com. Tel: +8628 85410957.
Abbreviations: DNA,
Deoxyribonucleic acid;
cDNA,
complementary DNA; Hsp, heat shock protein; PCR,
polymerase chain reaction; RT-PCR, reverse
transcriptase – PCR; CTAB, cetyl trimethyl ammonium
bromide; dCTP, deoxycytidine 5’- triphosphates;
SDS, sodium dodesyl sulfate; RE, relative
enrichment; EDTA, ethylene diamine tetraacetic acid;
PAGE, polyacrylamide gel electrophoresis
Accepted
7 January, 2010 |
|
DnaJ
(Hsp40), a heat shock protein, is a molecular chaperones
responsive to various environmental stress. To analyze the
protective role of DnaJ, we obtained sense transgenic
Arabidopsis plants that constitutively expressed elevated
levels of DnaJ. In this study, sense transgenic plants show
large thinner, fade color and malformed leaves, as well as
less floss of back leaves. Plants with enhanced levels of
DnaJ in their transgenic sense lines exhibited tolerance to
NaCl stress. Under 120 mM NaCl, root length was higher in
transgenic sense plants than wild-type plants.
In vitro
expression system, DnaJ protein shows tolerance to high NaCl.
These results suggest that over-expression of DnaJ can
confer NaCl-stress tolerance.
Key
words:
DnaJ (Hsp40), NaCl, sense-transgenic plants, in vitro
expression. |
