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Antioxidant activities of the selected plants from the
family Euphorbiaceae, Lauraceae, Malvaceae and Balsaminaceae
Durre Shahwar*, Shafiq-ur-Rehman, Naeem Ahmad, Sami Ullah
and Muhammad Asam Raza
Center for Natural Product Drug Development,
Department of Chemistry, Government College University
Lahore-54000, Pakistan.
*Corresponding author. E-mail:
drdshahwar@yahoo.com.
Tel: +92-333-4566845.
Accepted 18 January, 2010 |
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Extraction
of nine plants selected from the family Euphorbiaceae,
Lauraceae, Malvaceae and Balsaminaceae was done in petroleum
ether, chloroform, ethyl acetate and methanol/n-butanol in
order of increasing polarity using soxhlet apparatus. Total
phenolic contents were determined with Folin-Ciocalteu
reagent which ranged from 30.5 to 547.0 mg GAE/g of extract.
Maximum phenolic contents were found in n-butanol extract of
Ricinus communis. Antioxidant
activities of these extracts were evaluated through DPPH•
radical scavenging, phosphomolybdate and ferric thiocyanate
(FTC) methods. Methanolic extract of
Cinnamomum zeylanicum
and
Cinnamomum
tamala showed highest antiradical (96.8%) and
phosphomolybdate (1.131) activity, respectively, while ethyl
acetate extract of R. communis exhibited maximum
lipid per-oxidation (FTC) activity (79.3%). IC50
value of chloroform extract of C. tamala (2.2 μg/ml)
was less than gallic acid (4.4 μg/ml), while ethyl acetate
and methanol extracts of
Abutilon bidentatum,
Impatiens bicolor and
Impatiens edgeworthii exhibited the IC50
values in the range of 10.0 - 20.0 μg/ml.
Key
words:
Euphorbiaceae, Lauraceae, Malvaceae, Balsaminaceae,
antioxidant assays, medicinal plants, total phenols. |
