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Antimicrobial and phytochemical
analysis of leaves and bark extracts from Bridelia
ferruginea
Owoseni, Abimbola A.*, Ayanbamiji, T.
A., Ajayi, Yejide O. and Ewegbenro Ikeoluwa B.
Department
of Biological Sciences, Bowen University, Iwo, Osun State,
Nigeria.
*Corresponding author. E-mail:
tokawal@yahoo.com.
Tel: +234-803 325 0240.
Accepted 5
October, 2009 |
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Five
bacteria and three fungi were grown and tested against the
effects of crude ethanolic, methanolic and water extracts of
Bridelia ferruginea leaves and bark. The leaf
water extract had zero antimicrobial activity. The overall
antimicrobial activity recorded zones of inhibition ranging
between 9 and 20 mm. The crude extracts were not active
against the fungi tested. The minimum inhibitory
concentration (MIC) of the active extracts was determined.
Half strength (10 g/ml) concentrations of the bark ethanol
and methanol extracts were the MICs against Citrobacter
sp. and Bacillus subtilis. While quarter strength
(5 g/ml) concentrations of the bark methanol and ethanol
extracts were the MICs against Staphylococcus aureus
and Micrococcus luteus. The phytochemical
analysis carried out on B. ferruginea leaves and bark
detected the presence of alkaloids, flavonoids, tannin,
cardiac glycosides, anthraquinone, phlobatinnin and saponins
and was negative for anthocyanin.
Key
words:
Bridelia ferruginea, phytochemical analysis,
antimicrobial activity, zone of inhibition. |
