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Genetic
diversity of gliadin pattern, morphological traits and
baking quality in doubled haploid wheat
J. Ojaghi* and
E. Akhundova
Baku State University,
Baku, Azerbaijan.
*Corresponding author. E-mail:
javid_804@yahoo.com.
Accepted 11 January, 2010 |
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This study aimed at assessing the genetic diversity of 102
lines doubled haploid wheat (sent from CIMMYT) using acid-polyacrylamide
gel electrophoresis
(A-PAGE) method, morphological traits and baking quality.
Cluster analysis according to morphological traits divided
all genotypes into four groups, so that genotypes with high
yield were placed in one group. However, by cluster analysis
according to their qualitative traits, the genotypes were
classified into three main groups, while genotypes with
higher amount of protein was placed in a separate group. In
the studied lines, 48 bands and 47 different patterns were
detected and polymorphism was observed in most of the bands.
In the ω area, 18 bands and 19 different patterns were
observed and the most amount of band was observed in this
area. In γ and β areas, 12 and 9 bands, 19 and 12 patterns
were observed, respectively. The least pattern variety was
seen in α area, presumably because the bands did not
separate properly in the one-dimensional electrophoresis in
this area. Seven patterns and nine bands were observed in
this area. Using Nei formula and according to the patterns,
the genetic diversity for all four areas (α, β, γ, ω) was
calculated, according to which γ area with H = 0.872 had the
most genetic diversity, then came ω and β areas with H =
0.767 and H = 0.714, respectively, and the least genetic
diversity was observed in α area with H = 0.646. Cluster
analysis according to protein bands has classified genotypes
into 9 main groups. Although the lines studied in this
research had the same parents, considerable diversity was
observed among them. Therefore, the electrophoresis of
polyacrylamide gel of gliadins can be used as a strong
system for identifying similar varieties. While comparing
the observed patterns, one pattern in ω area was proved to
be relevant to the trait of the number of grain per
spikelet, which can be used as a marker in order to increase
yield.
Key words:
Genetic diversity, gliadin patterns, morphological traits,
backing quality, doubled haploid wheat. |
