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A comparative
study of chromosome morphology among some accessions of
Aegilops crassa
Hatami-Maleki
Hamid1, Samizadeh Habibollah2,
Asghari-Zakaria Rasool3, Moshtaghi Nasrin4
and Fazeli-Sangari Esmaeil4
1Department
of Agriculture and Plant Breeding, Faculty of Agriculture,
Maragheh University, Maragheh, Iran.
2Department
of Agriculture, Faculty of Agriculture, Guilan University,
Rasht, Iran.
3Department
of Agriculture and Plant Breeding, Faculty of Agriculture,
Mohaghegh Ardebili University, Ardebil, Iran.
4Department
of Biotechnology, Faculty of Agriculture, Ferdowsi
University of Mashhad, Mashhad, Iran
*Corresponding author. E-mail:
hatamimaleki@yahoo.com.
Accepted 10 April, 2009 |
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In this study karyotype and chromosome characteristics of
the nine accessions of Aegilops crassa species
obtained from gene bank of Seed and Plant Improvement
Research Institute (SPII) of Iran and one accession
collected by authors were inspected.
Aceto-iron-hematoxilin staining method was used to stain
chromosomes. For each accession, chromosome characteristics
including long and short arms, chromosome lengths, arm ratio
index and relative chromosome lengths were measured using
micro measure 3.3 software.
Results revealed that all of the studied accessions were
tetraploid (2n = 4x = 28) and consisted of 13 pairs of
metacentric and one pair of submetacentric chromosomes, of
which two pairs were satellite chromosomes. Karyotype
formula for these accessions were 13 m + 1 sm.
Arm ratio index value of chromosomes ranged from 1.11 in
chromosome number 6 to 1.77 in chromosome number 12. The B
chromosomes were not seen in any of the
accessions.
Karyological characteristics of
these
accessions were similar to each other. However, some
differences were observed between the accessions in some
chromosome characteristics.
Key words:
Aegilops Crassa, karyotype, aceto-iron-hematoxilin staining, satellite chromosome. |
