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Sampling strategy to
develop a primary core collection of apple cultivars based
on fruit traits
Zhang Jie1,
Wang Yi1, Zhang Xinzhong1, Li
Tianzhong1, Wang Kun2, Xu Xuefeng1
and Han Zhenhai*
1Institute
of Horticultural Plants, China Agricultural University,
a
Key Laboratory of Beijing Municipality of Stress Physiology
and Molecular Biology for Fruit, Tree, Beijing 100193,
China.
2Fruit
Tree Research Institute of the Chinese Academy of
Agricultural Sciences, China.
Corresponding
author. E-mail:
rschan@cau.edu.cn.
Accepted
8 May, 2009 |
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A total
of 435 accessions of apple germplasm collected from the
Xincheng National Apple Germplasm Repository and 10
morphological traits of them were used for studying the
optimal sampling strategy for primary core collection of
apple (Malus domestica Brokh). In order to acquire
the appropriate primary core collection, different
entire sampling ratio and sampling scheme were compared in
the study. Six entire sampling ratios were tested and the
sampling schemes following stratification into two levels,
including the grouping principle, sampling proportion within
group were studied. The results showed that 10% should be
the suitable entire sampling ratio for primary core
collection of apple. Under 15% entire sampling ratio, the
optimal sampling scheme was grouped based on cultivar group
combining with genetic diversity based sampling proportion
within group proportion. This sampling strategy was used to
acquire the primary core collection of 64 accessions from
435 accessions of apple cultivars, and the primary core
collection could well represent the genetic diversities of
the entire variety collection.
Key
words:
Core
collection, sampling strategy, fruit traits,
apple. |
