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Cellulase production by
Trichoderma sp. on apple pomace under solid state
fermentation
Haiyan Sun1,
Xiangyang Ge2, Zhikui Hao2 and Ming
Peng1*
1Key
Laboratory of Tropical Crop Biotechnology, Ministry of
Agriculture, Institute of Tropical Bioscience and
Biotechnology, Chinese Academy of Tropical Agricultural
Sciences, Haikou 571101, China.
2The
Key Laboratory of Industrial Biotechnology, Ministry of
Education, School of Biotechnology, Jiangnan University,
China.
*Corresponding author.
E-mail:
mmpeng_2000@yahoo.com.
Tel.: +86-898-66963161. Fax: +86-898-66890978.
Accepted 4 June, 2009 |
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The
feasibility of using apple pomace for cellulase production
by Trichoderma sp. under solid state fermentation was
evaluated in this study. Our results indicated that initial
moisture level of the medium, incubation temperature and
inoculum size influenced the cellulase production greatly.
The optimum initial moisture level, incubation temperature
and inoculum size were 70%, 32 and 2×108
spores/flask, respectively. The supplement of lactose and
corn-steep solid to the apple pomace favored the enzyme
formation markedly.
Key
words:
Apple pomace, cellulase, Trichoderma sp. |
