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Evaluation of wheat by
polyacrylamide gel electrophoresis
Muhammad Shuaib1*, Mohsin Jamal1,
Haji Akbar1, Israr Khan1 and Rehmana
Khalid2
1Department
of Biotechnology, University of Malakand, Chakdara,
Pakistan.
2Department
of Chemistry, Lahore College for Women University, Pakistan.
*Corresponding author. E-mail:
shuaibm@yahoo.com
Tel: ++92 (945) 764131- (ext 3025). Fax: ++92 (945) -763491.
Accepted 3
September, 2009 |
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In this study 15 wheat varieties namely Nacozari-76, Ouqab,
Tatar-96, Bakhtawar-92, Yecura-70, Raj, Bakkar, Sulilman-96,
Pirsabak-05, Maria, Khyber-87, Fakhr-e-Sarhad, Pirsabak-04,
Inqulab-91 and Rawal-87 were evaluated for analysis of
variability in seed storage proteins by using sodium dodecyl
sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Electrophorogram for each variety
were scored and presence or absence of each band noted and
was entered in a binary data matrix. Based on the data of
SDS-PAGE gels cluster analysis was performed to check the
variations among varieties. The overall result shows low
degree of heterogeneity however different varieties reveal
differential protein banding pattern. It is concluded
that SDS-PAGE analysis of wheat endosperm protein is useful
for evaluation of genetic variability and cultivars
identification that help in wheat breeding program.
Key
words:
SDS-PAGE, glutenin proteins, wheat varieties, genetic
variation and cluster analysis (UPGMA). |
