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Interaction of IBA and NAA
with enzymes in root induction of Crocus sativus (L)
Golandam Sharifi and
Hassan Ebrahimzadeh*
Plant Physiology Laboratory Department of Botany,
School of Biology, College of Sciences, University of
Tehran, P. O. Box: 14155-6455, Tehran, Iran.
*Corresponding author. E-mail:
golandam@khayam.ut.ac.ir or
golandsharifi@gmail.com.
Abbreviations:
IBA, Indole-3- butyric acid; NAA,
α-naphthalene acetic acid; NBT, nitro blue
tetrazolium; PMS, phenazine metho sulphate; CAT,
catalase; PPO, polyphenoloxidase; PRX,
peroxidase; EST, esterase; LDH, lactate
dehydrogenase and L-DOPA, L-dihydroxy-phenyl-alanine.
Accepted
23 April, 2009 |
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Effect of
auxin type on some antioxidative enzymes during in vitro
root induction of saffron (Crocus sativus L.) was
investigated to reveal the variation of activities and
isoenzyme banding patterns during root induction of saffron
(C. sativus L.). Indole-3- butyric acid (IBA) and
naphtalene acetic acid (NAA) at different concentrations
were added to the solidified MS or B5 medium. Corm explants
of saffron were placed on root inducing media and enzyme
levels in explants prior or after adventitious root
formation were measured and compared. Percentage of root
formation on B5 medium containing 2.46 µM IBA was highest;
however the maximum number of root per explant and per cm2
observed on MS medium with 19.6 µM IBA. Significant changes
occurred in the activities of these enzymes. Some
polymorphisms occurred in patterns of lactate dehydrogenase
and esterase during root induction in different treatments.
Keywords: Crocus
sativus L., saffron, root formation, IBA, NAA. |
