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Full Length Research Paper
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Development of rapid,
specific and sensitive
detection of Cucumber mosaic virus
Haggag Salah Zein1*
and Kazutaka Miyatake2
1Department
of Genetics, Faculty of Agriculture, Cairo University,
12613, Giza, Egypt.
2Department
of Applied Biological Chemistry, Graduate School of
Agriculture and Biological Sciences, Osaka Prefecture
University, 1-1, Gakuen-Cho, Sakai, Osaka, 599-8531, Japan.
*Corresponding author: E-mail:
haggagsalah@gmail.com.
Tel: +20128436419 +2025685148. Fax: +2025717355,
+2025720739.
Accepted
23 November, 2007 |
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Abstract |
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Cucumber mosaic
virus (CMV) causes major losses to thousand agricultural and
horticultural crops around the world. Unlike other plant
pathogens, there are no direct methods available yet to
control viruses and, consequently, the current measures rely
on indirect tactics to manage the viral diseases. Hence,
methods for detection and identification of viruses, both in
plants and vectors, play a critical role in virus disease
management. A rapid assay for diagnosis of CMV, which can be
employed in both laboratory and field, is essential.
Therefore, this study was undertaken to develop a procedure
for detection of the CMV in infected plants using a
monoclonal and polyclonal antibodies. Dot-immunobinding
assays (DIBA) are useful alternatives to microtitre plate
enzyme-linked immunosorbent assay (ELISA). Nine monoclonal
antibodies were readily used for detected CMV by TAS-ELISA
and DIBA of infected plants. DIBA has about the same
sensitivity as ELISA in microtiter plates, but it has the
additional advantages of simplicity, quick completion in the
field or office on large numbers of samples, economy, and
can be quantified using densitometry.
Key
words:
Monoclonal antibodies, polyclonal antibodies,
triple-antibody sandwich, enzyme-linked immunosorbent assay,
dot-immunobinding assays. |
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