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Morphology and cytology of
flower chimeras in hybrids of Brassica carinata ×
Brassica rapa
Jianmin Liu1,3, Hao Wang2,
Longjiang Yu1,3, Dianrong Li2*
and Maoteng Li1,3*
1Key
Laboratory of Molecular Biophysics, Ministry of Education,
Huazhong University of Science and Technology, Wuhan 430074,
China.
2Hybrid Rapeseed Research Center of Shanxi Province, Dali
715105, China.
3College
of Life Science and Technology, Huazhong University of
Science and Technology, Wuhan 430074, China.
*Corresponding author. E-mail:
limaoteng426@163.com
or
lidr@peoplemail.com.cn.
Accepted
15 January, 2009 |
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Hybridization between white flowered Brassica carinata
and yellow flowered B. rapa were made,
and the
flower chimeras were observed in a few hybrids. The simple
single sequence
molecular markers verified the hybridity
of those hybrids.
Chimeras
were justified and totally classified based on the
morphological characteristics of the flower petals that
appeared in the hybrids of B. carinata and B. rapa.
Two
kinds of flower chimeras were observed: one type was
different flower petals were with different colour in one
branch; another type was that yellow petals were with white
variegations, but the variegation size and shape were
different in different petals. The meiosis and mitosis
analysis showed that the partial or complete separation of
parental genomes inferred to occur in pollen mother cells,
shoot and early-developed petals in the flower chimeral
hybrids, which hinted that the occurrence of complete or
partial segregation of parental genomes in the somatic cells
might be the reason for the production of flower chimera in
the hybrids of B. carinata and B. rapa.
Key
words:
Brassica carinata, B. rapa, SSR, flower
chimera. |
