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Endophytic fungi from
Dracaena cambodiana and Aquilaria sinensis and
their antimicrobial activity
Li-Juan Gong and Shun-Xing Guo*
1Institute
of Medicinal Plant Development, Peking Union Medical College
and Chinese Academy of Medical Sciences, Beijing, 100193,
China.
*Corresponding author. E-mail:
sxguo2006@yahoo.com.cn. Tel: +86-10-62829619. Fax:
+86-10-62895120.
Accepted
8 October, 2008 |
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Single mycelium method was used to isolate endophytic fungi
from
surface-sterilized tissues of two medicinal plants.
Of the
300 isolates recovered, 172 were from
Dracaena
cambodiana
and 128 from
Aquilaria
sinensis.
According to morphological
characteristics, 174 (58%) isolates were identified and
belonged to 41 taxa in 30 genera. The remaining 126 (42%)
isolates did not sporulate and were segregated into 8
morphological groups.
There
were colonization rates
(34
- 80%)
and isolation rates (0.62 - 2.06) in two plants. And some
endophytes showed certain level of host specificity or
organs specificity.
21 (8.3%)
isolates showed antimicrobial
activity, moreover, some of them exhibited broad-spectrum
antimicrobial
activity
and the inhibition zones ranged from 7 to 27 mm.
The active isolates were identified to 17 taxa.
Fusarium spp. were the most dominant genera in two
plants and showed the most potent antimicrobial activity.
Key
words:
Antimicrobial activity, Aquilaria sinensis, Chinese
medicinal plants, Dracaena cambodiana,
endophytic fungi. |
