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Complete nucleotide
sequencing, SNP identification and characterization of SRY
gene in Indian Sangamneri goat
Prashant, D. S. Gour, P. P. Dubey, A. Jain, D. K. Nanda,
B. K. Joshi and Dinesh Kumar*
Genes and
Genetic Resources Molecular Analysis Lab, National Bureau of
Animal Genetic Resources, Karnal –132001, India.
*Corresponding author. E-mail:
dineshkumarbhu@gmail.com.
Accepted 27
March, 2009 |
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The SRY gene is responsible for testis determination in
mammals. It codes for a protein with a DNA binding domain
similar to those found in high-mobility group protein (HMG
box). Mutation in this gene leads to Gonadal Dysgenesis an
abnormal sexual phenotype described in human, cattle, horse,
and river buffalo. Here we report PCR-SSCP analysis and
sequencing of coding region of SRY gene in Sangamneri
goat (Capra hircus) using 40 male goats (bucks)
samples. Polymorphism was detected in only one sample
indicating that a major portion of SRY gene is highly
conserved in this breed.
Key words:
Goat, HMG box, SSCP, SRY gene. |
