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Isolation of
high quality DNA and RNA from cambium of the East African
Greenheart (Warburgia ugandensis)
E. Muge1*,
K. Burg2, C. Kadu1, A. Muchugi1,
S. Lemurt1 and R. Jamnadass1
1World
Agro forestry Centre (ICRAF), Box 30677-00100, Nairobi,
Kenya.
2Austrian
Research Centers GmbH - ARC Department of Bioresources,
A-2444 Seibersdorf, Austria.
*Corresponding author. E-mail:
e.kirwa@cgiar.org.
Tel.: +254-20-7224000.
Fax: +254-20-7224001.
Abbreviations:
CTAB, Cetyl-trimethylammoniumbromide; RT-PCR,
reverse-transcription-polymerase chain reaction; PVP,
Polyvinylpyrrolidone; DEPC, diethylpyrocarbonate.
Accepted 14 May, 2009 |
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The genus Warburgia belongs to the Canellaceae,
a small family of tropical aromatic tree species important
for their antibacterial and antifungal medicinal qualities
amongst many other functions. Many published protocols for
nucleic acid isolation failed to yield sufficiently good
quality amounts for analysis. We have therefore developed a
simple and fast CTAB (cetyl-trimethyl ammonium
bromide)-based extraction protocol for routine isolation of
high-quality nucleic acids. Good quality genomic DNA (gDNA)
and RNA was obtained and was suitable for PCR amplification,
restriction endonuclease digestion, Southern blotting and
RT-PCR, respectively.
Key words:
Nucleic acid extraction, polyphenols, polysaccharides,
medicinal plants. |
