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Study of
BMP-15 gene polymorphism in Iranian goats
Hamid
Deldar-Tajangookeh1*, Ahmad Zare Shahneh1,
Mohammad Javad Zamiri2, Morteza Daliri3,
Hamid Kohram1 and Ardeshir Nejati-Javaremi1
1Department
of Animal Science, Faculty of Agriculture, University of
Tehran, Karaj, Iran.
2Department
of Animal Science, College of Agriculture, Shiraz
University, Shiraz, Iran.
3Department
of Microbiology, National Institute for Genetic Engineering
and Biotechnology, Tehran, Iran.
orresponding author. E-mail:
hamiddeldar@gmail.com.
Fax: +98-2612246752.
Tel.: +98-2612248082.
Accepted 5 January, 2009 |
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Different mutations in the bone morphogenetic protein-15 (BMP-15)
and the Growth Differentiation Factor-9 (GDF-9) genes
have increased ovulation rate and infertility in a
dosage-sensitive manner in sheep. To test the polymorphisms
of genes in goat, which have been demonstrated as major
genes of fecundity in sheep, the genetic polymorphism of
FecXB and FecXG loci in
BMP-15 gene were studied in 109 Iranian native goats.
Blood samples were collected in EDTA coated tubes from
Jugular vein and genomic DNA was extracted from whole blood
samples. Single nucleotide polymorphism of FecXB
and FecXG loci in BMP-15 gene were
determined using PCR-RFLP technique. There was no evidence
of mutation in FecXB and FecXG
in these goats, all of which were monomorph for exon 2
BMP-15 gene.
Key words:
Goat, FecXB, FecXG,
BMP-15, polymorphism. |
