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Screening for
lipase-producing Enterobacter agglomerans for
biodiesel catalyzation
Zhang Zhen-qian and Guan Chun-yun*
Oilseed
Research Institute, Hunan Agricultural University, Changsha,
410128,
P. R.
China.
Corresponding
author. E-mail:
guancy2000@yahoo.com.cn. Fax: 86-0731-4618778.
Accepted
22 December, 2008 |
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This paper reports a comprehensive study of screening
rapeseed soil sample in Victoria
blue solution solid medium, with
olive oil as sole carbon source. The
lipase of Enterobacter
agglomerans which has the highest activity
was identified. Under the optimum
conditions determined, the enzyme activity of this strain
reached 39.09 U/mL. The optimal conditions were found to be:
initial pH 7.0, agitating at 30°C for 48 h, with 2.0% (w/v)
lactose as carbon source, 1.5% (w/v) beef extract and 1.0%
(w/v) yeast extract
as nitrogen source, and
0.1% (w/v)
MgSO4·7H2O. Olive oil, sesame oil and
tea oil as raw materials can be catalyzed to biodiesel by
the lipase of this strain at 30°C and 180 rpm. And the yield
reached 54.51% with sesame oil as raw material, even when
they contained 92.4% (w/v) water in the starting materials.
This strain will potentially serve as a promising
alternative lipase for biodiesel production with raw
materials containing water.
Key
words:
Biodiesel, lipase, enzyme activity, culture condition. |
