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  Afr. J. Biotechnol.

  Vol. 8 No. 7

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  Amadi JE
  Adeniyi DO

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African Journal of Biotechnology Vol. 8 (7), pp. 12191221, 6 April 2009

ISSN 1684-5315  © 2009 Academic Journals  

 

 

Full Length Research Paper

 

Mycotoxin production by fungi isolated from stored grains

 

Amadi, J. E.* and Adeniyi, D. O.

 

Department of Plant Biology, University of Ilorin, P.M.B. 1515, Ilorin, Nigeria.

 

*Corresponding author. E-mail: jamadi2007@yahoo.com. Tel: 08033710308.

 

Accepted 2 January, 2009

 
   Abstract
 

A study was conducted to determine the fungi associated with maize (Zea mays), rice (Oryza sativa) and millet (Pennisetum typhoiodes) in storage. Mycotoxin production by isolated fungi was subsequently evaluated using the thin layer chromatography technique. Eight different fungi were isolated altogether namely Aspergillus terreus, Aspergillus flavus, Aspergillus niger, Aspergillus oryzae, Penicillium italicum, Penicillium spinulosum, Rhizopus stolonifer and Fusarium sp. The results of this study show that all the fungi produced one toxin or the other as detected in the culture filtrates of isolated fungi. However, only three of these toxins were identified namely aflatoxin B1, fumonisin B1, and zearalenone. The retention factors (Rf) of all the toxins produced were determined.

 

Key words: Maize, rice, millet, storage fungi, mycotoxins.

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