Full Length Research Paper

Trino IB ameliorates the oxidative stress of cryptorchidism in the rat

A.O. Afolabi¹*, O. Aluko¹, O. Oyewopo², B.M. Olabinri³, O. Olotu¹ and Y. Raji⁴

¹Physiology Department, College of Health Sciences, LAUTECH, Ogbomoso, Nigeria.

²Anatomy Department, College of Health Sciences, LAUTECH, Ogbomoso, Nigeria.

³Biochemistry Department, College of Health Sciences, LAUTECH, Ogbomoso, Nigeria.

⁴Physiology Department, College of Medicine, UNIBADAN, Ibadan, Nigeria.

*Corresponding author. E-mail: oasafolabi@yahoo.com.

Accepted 5 December, 2008

The study examined the effect of Trino IB, a mixture of oleuropein and α-lipoic acid, on the plasma concentration of g-glutamyl transpeptidase (GGT), malondialdehyde (MDA), sperm count and apoptotic degeneration of testicular germ cells in experimentally induced cryptorchidism in adult male rats. Oral administration of Trino IB (0.5 ml/kg body weight) for 56 days to rats caused significant reduction (P<0.05) in the plasma concentration of GGT in the treated groups, but no significant difference (P=0.0839) in the plasma MDA concentration was observed. Histologically, Trino IB administration significantly elevated (P<0.05) the number of sperm cells in the treated cryptorchid groups compared to the untreated cryptorchid groups. Trino IB alleviated apoptotic degeneration of germ cell in the testes of the treated groups, while there was apoptotic degeneration in the testicular germ cells of the untreated groups. Trino IB alleviated the deleterious effect of oxidative stress in induced cryptorchidism.

Key words: Cryptorchid, oxidative stress, Trino IB, g-glutamyl transpeptidase, malondialdehyde.