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Establishment of the
callus and cell suspension culture of Elaeagnus
angustifolia for the production of condensed tannins
Fan-Suo Zeng1, 2,
Wei-Wei Wang2, Ya-Guang Zhan1,2* and
Ying Xin3
1Key
Laboratory of Forest Tree Genetic Improvement and
Biotechnology, Ministry of Education, Northeast Forestry
University, Harbin 150040, China.
2College
of Life Science, Northeast Forestry University, Harbin
150040, China.
3College
of forestry, Northeast Forestry University, Harbin 150040,
China.
*Corresponding author. E-mail:
youpractise@126.com.
Abbreviations: PGR,
plant growth regulator; DW, dry weight; FW,
fresh weight; 2,4-D, 2,4-dichlorophenoxyacetic acid;
BA, 6-bezyladenine; NAA, α-naphthaleneacetic
acid; IAA, Indole-3-acetic acid.
Accepted
2 July, 2009 |
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The
objective of this work was the optimization of the
conditions of callus and cell suspension culture of
Elaeagnus angustifolia for the production of condensed
tannins. The effects of different conditions on the callus
growth and the production of condensed tannins were
researched. The leaf tissue part of E. angustifolia
was optimum explant of callus induction. The best callus
growth and the highest condensed tannins production was
obtained under the culture on MS basal medium containing
1.00 mg·L-1 2,4-D and 0.50 mg·L-1 BA.
The optimum time of subculture was 20 - 25 d. The results
showed the contents of condensed tannins decreased gradually
at the beginning of the callus differentiation.
Vigorous
and
friable
callus
was used for cell suspension culture.
Study on the
effect of medium and the
hormone combination
showed
that plenty of incompact and rapid growing suspension cells
were obtained from cultures grown in liquid medium
supplemented with 0.1 mg·L-1
BA + 0.1 mg·L-1
TDZ.
The study
provided an efficient way for E. angustifolia cell
suspension culture to produce secondary metabolite.
Key
words:
Elaeagnus angustifolia L, callus, condensed tannins,
suspension culture. |
