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The effect of vitamin C
and cobalt supplementation on antioxidant status in healthy
and diabetic rats
Özlem Yıldırım
Ankara University, Faculty of Science, Department of
Biology, 06100, Tandogan, Ankara, Turkey. E -mail:
yildirim@science.ankara.edu.tr. Tel: +90-312-212-6720
ext. 1090. Fax: +90-312-223-2395.
Accepted 18 August, 2009 |
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In the
present study, liver, lung, heart and kidney superoxide
dismutase (SOD), glutathione peroxidase (GSH-Px), catalase
(CAT) activities, lipid peroxidation, nitrite and vitamin C
levels were investigated in diabetic rats. Diabetes induced
in rats by streptozotocin (STZ) and the treated rats were
received 1 g/l vitamin C with 0.5 mM CoCl2 in
drinking water for eigth weeks. In all tissues, superoxide
dismutase, glutathione peroxidase, catalase activities,
lipid peroxidation and nitrite levels were significantly
increased in diabetic rats at the end of 8th week
(p < 0.05) whereas vitamin C level was decreased compared to
those of controls. Cobalt with vitamin C treatment of
diabetic rats resulted in partial restoration of SOD and CAT
activities, thiobarbituric acid reactant substances (TBARS),
vitamin C and nitrite levels at all times studied, whereas
treatment did not significantly change GSH-Px activity
compared to diabetics. These results suggest that cobalt
with vitamin C, effectively but could not completely restore
the altered endogenous defense systems in diabetic rat
liver, lung, heart and kidney tissues.
Key
words:
Cobalt, antioxidant enzymes, diabetes, lipid peroxidation,
tissue. |
