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Recent advance in the
support and technology used in enzyme immobilization
Tian Xie*, Anming Wang,
Lifeng Huang, Haifeng Li, Zhenming Chen, Qiuyan Wang and
Xiaopu Yin
Research Center for Biomedicine and Health, Hangzhou
Normal University, No. 222, Wenyi Road, Hangzhou 310012, P.
R. China
*Corresponding author. E-mail:
tianxiehz@hotmail.com.
Tel.: +86-571-28861623. Fax: +86-571-28865630.
Accepted 4 September, 2009 |
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In the
industrial biotechnology, the skill to prepare the
immobilized enzyme with stability has proven to be one of
the key steps in rendering an enzymatic process that is
economically viable. Availability of the immobilized enzyme
biocatalyst with improved activity and stability is also
expected to reduce the expense of products. If properly
designed, enzyme immobilization is also a very powerful tool
to enhancing almost all enzyme properties, e.g., activity,
stability, specificity and selectivity, reduction of
inhibition. Due to the above advantages, enzyme
immobilization has been a focus for biologists, chemists and
enterprisers. This review is focused on advances over the
last several years in enzyme immobilization. Several new
type of carriers and technology have been implemented to
improve traditional enzyme immobilization, which aimed to
enhance enzyme loading, activity and stability to decrease
the enzyme biocatalyst cost in industrial biotechnology.
These include cross-linked enzyme aggregates (CLEAs),
microwave-assistant immobilization, click chemistry
technology, mesoporous support and single enzyme
nanoparticle. In addition, with the growing attention paid
to cascade enzymatic reaction and in vitro synthetic
biology, it is possible that multi-enzyme co-immobilization
would be one of the next goals in the future.
Key
words:
Enzyme immobilization, cross-linked enzyme aggregates (CLEAs),
microwave irradiation,
mesocellular siliceous foams (MCFs),
click
chemistry, single enzyme nanoparticle. |
