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Genetic diversity of
Iris lactea var. chinensis germplasm detected by
inter-simple sequence repeat (ISSR)
Kang Wang1, 2,
Junmei Kang2, He Zhou1*, Yan Sun1,
Qingchuan Yang2, Jie Dong2 and Lin
Meng3
1Institute
of Grassland Science, China Agricultural University,
Beijing, 100193, People’s Republic of China.
2Institute
of Animal Science, Chinese Academy of Agricultural Sciences,
Beijing, 100193, People’s Republic of China.
3Beijing
Research Development Center for Grass and Environment,
Beijing, 100097, People’s Republic of China.
*Corresponding author. E-mail:
zhouhe@cau.edu.cn.
Tel/Fax: 86-10-62734122.
Accepted 31 August, 2009 |
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The
genetic diversity of 24
Iris lactea
var. chinensis
were
analysed by using 100 ISSR primers of which only 11 primers
generated distinct amplified products.
Among the 24 materials, a total of 214 bands were detected,
of which 170 bands were polymorphic. The percentage of
polymorphic bands (PPB) was 79%.
The ISSR-based
genetic similarity (GS) values among 24 accessions ranged
from 0.400 to 0.929. Abundant genetic diversity among I.
lactea var. chinensis accessions was revealed by
ISSR markers. Based on the UPGMA cluster analysis and
principal components analysis (PCA), the 24 materials investigated were divided into four
groups, most of genetic variation was within a group.
Genetic distance was found to be related to geographic
distance among I. lactea var. chinensis.
Key
words:
Iris lactea var. chinensis,
inter-simple sequence repeat (ISSR) marker, genetic
diversity. |
