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The effect of plant growth
regulators, cultivars and substrate combination on
production of virus free potato minitubers
Taha Roodbar Shojaei1*,
Niaz Ali Sepahvand2, Mansour Omidi3,
Hamid Reza Abdi2 and Sepehr Mohajeri Naraghi4
Department
of Agronomy and Plant Breeding, Faculty of Agriculture,
Islamic Azad University, Karaj Branch, Karaj, Iran.
Seed
and Plant Improvement Institute, Karaj, Iran.
3Department
of Agronomy and Crop Breeding, College of Agriculture,
University of Tehran, Karaj, Iran.
4Young
Researchers Club, Islamic Azad University, Karaj Branch,
Karaj, Iran.
*Corresponding author. Email:
tahashojaee2000@yahoo.com. Tel.:+989161112856.
Abbreviations: MS,
Murashige and skoog; BA, butyric acid; IBA,
indol butyric acid; NAA, naphthalene acetic acid;
GA3, gibberlic acid; BAP, (6-benzyl-amino purine;
KIN,
6-furfuryl amino purine;
DAS-ELISA, double antibody sandwich ELISA; CRD,
completely randomized design.
Accepted 16 July, 2009 |
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Nowadays
large amounts of potato seed in the world is produced by
in vitro virus free minitubers. Therefore, evaluation of
commercial varieties in production of virus free potato
minitubers is critical. In this study virus free plantlets
of 4 potato cultivars of Agria, Marfona, Sante and Burren
were achieved with meristem culture method. The meristem-derived
plantlets which had optimum growth were transferred to the
MS-medium without any growth regulators to stimulate the
same vigor of the plantlets. Then DAS-ELISA test was
conducted and the effects of different level of
2 growth regulators either singly or in combination
were evaluated on single node culture of potato. In all
cultivars the best and most economical medium for single
node culture was MS medium without growth regulators.
Afterwards virus free plantlets were selected and
transferred to the green house. The effects of substrate
combination including 4 planting bed in minituber production
were evaluated, after 90 days, numbers and diameters of
minitubers for each cultivar were counted and recorded.
Marfona had the greatest number of minitubers and Agria had
the lowest. There are no any significant differences in
minituber diameter between the cultivars. A mixture of peat
moss and sand in the ratio of 1:1 was proved to be the best
for minituber production.
Key
words:
Potato, plant growth regulators, single node culture,
substrate combination, minituber. |
