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Effect of curcumin on
Helicobacter pylori biofilm formation
Panan Pattiyathanee1,2, Ratha-korn Vilaichone3
and Nuntaree Chaichanawongsaroj1*
1Department
of Transfusion Medicine, Faculty of Allied Health Sciences,
Chulalongkorn University, 154 Rama I road, Pathumwan,
Bangkok, 10330 Thailand.
2Inter-department
of Biomedical Sciences, Faculty of Graduated School,
Chulalongkorn University, 10300 Thailand.
3Division
of Gastroenterology, Department of Medicine, Thammasat
University, 12120 Thailand.
*Corresponding author. E-mail:
Nuntaree.c@chula.ac.th
or nuntaree@gmail.com.
Tel: 66-2-2181084. Fax: 66-2-2181083.
Abbreviations: VBNC,
Viable but non culturable; BHI, Brain heart infusion;
BCD,
b-cyclodextrin.
Accepted 27
August, 2009 |
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Helicobacter pylori
is a leading
etiologic
agent causing peptic ulcer
and gastric cancer. The alternative lifestyle as a
biofilm facilitates H. pylori
to survive in adverse environments.
Here, we investigated effect of curcumin
on H. pylori biofilm formation both qualitatively by
pellicle assay and quantitatively by crystal violet
staining. Three-dimensional
structure of biofilm
was imaged by scanning electron microscopy. The effect of
curcumin on H. pylori adherence to HEp-2 cells was
also investigated. Sub-inhibitory concentrations of curcumin
inhibited the biofilm in dose dependent manner. However,
H. pylori could restore ability to form biofilm during
extended time of incubation. Scanning electron microscopy
revealed less amorphous extracellular polymeric matrix, slow
of morphological conversion to coccoid form with cell damage
after curcumin treatment. Curcumin significantly decreased
the ability of H. pylori to adhere to the HEp-2
cells. Our findings demonstrated advantages of curcumin to
inhibit biofilm formation by H. pylori, making it as
a potential complimentary medicine for curing of H.
pylori-biofilm related infections.
Key words:
Helicobacter pylori, biofilm, curcumin, adhesion,
scanning electron microcopy, sub-inhibitory concentrations. |
