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Comparative study of
artesunate, ACTs and their combinants on the biochemical
parameters of male guinea-pigs
A. W. Obianime and J. S.
Aprioku*
Department of Pharmacology, Faculty of Basic Medical
Sciences, College of Health Sciences, University of Port
Harcourt, P. M. B. 5323, Port Harcourt, Rivers State, Nigeria.
*Corresponding author. E-mail:
sydaprio@yahoo.com.
Tel.: +2348035082379.
Abbreviations:
ACT, Artemisinin-based combination therapy; ALP,
alkaline phosphatase; ACT, total acid phosphatase;
ACPP, prostatic acid phosphatase; GP, guinea-pig.
Accepted
17 August, 2009 |
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In this study, the effects of different doses of
artesunate, artesunate/sulfadoxine/ pyrimethamine,
artesunate/amodiaquine and their combinants
(sulfadoxine/pyrimethamine and artesunate/ amodiaquine) on
the biochemical parameters- alkaline phosphatase (ALP),
total acid phosphatase (ACPT), prostatic acid phosphatase
(ACPP), urea, creatinine, uric acid and total cholesterol of
the male guinea-pig were investigated. Basal serum ACPP
value was increased from 3.50 ± 0.42 to 4.75 ± 0.85 and 4.75
± 0.48 IU/L by amodiaquine and sulfadoxine/pyrimethamine
respectively at their subclinical doses. These values were
significant at p < 0.05. Furthermore, the agents caused no
significant effects (p < 0.05) on serum ALP and ACPT.
Artesunate/sulfadoxine/pyrimethamine significantly increased
(p < 0.05) serum urea at the 3 doses, while the effects of
artesunate and artesunate/amodiaquine were biphasic,
decreasing basal serum urea level at lower doses and
increasing at higher doses. However, basal serum creatinine
level was increased from 62.5 ± 6.7 to 89.8 ± 4.8 µmol/L by
sulfadoxine/ pyrimethamine and decreased to 53.0 ± 0 µmol/L
by artesunate/amodiaquine at their subclinical doses, while
the other agents caused no significant (p < 0.05) effects.
Furthermore, control serum uric acid level was significantly
decreased from 368.8 ± 10.0 to 318.3 ± 5.8, 306.0 ± 1.2 and
312.3 ± 2.6 mmol/L by artesunate, amodiaquine and
artesunate/amodiaquine, after administration of half their
clinical doses, respectively. Total blood cholesterol was
also significantly reduced from 2.43 ± 0.13 to 2.1 ± 0 and
1.9 ± 0 mmol/L after administration of clinical doses of
amodiaquine and artesunate/amodiaquine, respectively. These
biochemical changes may be due to oxidative effects induced
by these agents.
Key words:
Biochemical, phosphatase enzymes, prostatic acid
phosphatase, ACT, combinants. |
