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Phenotypic and molecular
evaluation of genetic diversity of rapeseed (Brassica
napus L.) genotypes
Marjanovic-Jeromela Ana,
Kondic-Spika Ankica*, Saftic-Pankovic Dejana, Marinkovic
Radovan and Hristov Nikola
Institute of Field and Vegetable Crops, M. Gorkog 30, 21000
Novi Sad, Serbia.
*Corresponding author. E-mail:
ankica@ifvcns.ns.ac.yu. Tel.: +381 21 4898 224. Fax:
+381 21 4898 222.
Accepted 24 August, 2009 |
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The genetic
diversity and relationships among rapeseed genotypes were
evaluated using quantitative analysis and random amplified
polymorphic DNA (RAPD) markers. Ten morphological and
biochemical traits (plant height, height to the first branch,
stem diameter, branch number, leaf number, pod number, seed
yield per plant, 1000-seed weight, oil content and protein
content) were analyzed in a three-year field experiment with
30 winter rapeseed genotypes. RAPD analysis was performed
with 13 primers, chosen according to previous results.
Significant genetic variability among the genotypes was
obtained at both the morphological and molecular level. Nine
out of the thirteen RAPD primers that were used for the
analysis enabled the estimation of DNA polymorphism of the
genotypes, while the other primers were monomorphic.
Dendrograms obtained for morpho-biochemical traits and for
the RAPD results were compared. In both cases partial
grouping of genotypes based on their geographical origin was
established.
Key
words:
Brassica napus
L., genetic diversity, RAPD, cluster analysis. |
