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Phylogenetic relationships
within indigenous Sudanese Cassia senna (L.) using
RAPD molecular markers
Nada B. Hamza*, Rami S.
Habeballa and Isra E. Abdalla
Department of Molecular Biology, Commission for
Biotechnology and Genetic Engineering, National Centre for
Research, Khartoum, P. O. Box 2404, Sudan.
*Corresponding author. E-mail:
nada.hamza@gmail.com.
Tel: +249 922550236.
Accepted 20 August, 2009 |
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Random
amplified polymorphic DNA (RAPD) markers were used to assess
genetic diversity in Cassia senna (L.). RAPD
technique was carried out in a set of 27 (C. senna
(L.) genotypes collected from different regions of Sudan. A
total of 27 polymorphisms (5.2 polymorphic markers per
primer) out of 31 reproducible products (7.5 fragments per
primer) were obtained from the 6 primers used. The number of
bands per primer ranged from 4 to 7, whereas the number of
polymorphic bands ranged from 3 to 6, corresponding to 60%
of the amplification products. Low level of genetic
similarity was observed in the collected genotypes. Six
random primers, each with 10 bases generated a total of 27
polymorphic bands out of the 31 total bands, that is,
polymorphism of 60% was observed.
Molecular variance (STATISTCA) was used to
investigate the genetic diversity of the populations. UPGMA
clustering resulted in two major clades. It was concluded
that high level of polymorphism found suggests that RAPD
techniques can be useful for studying C. senna.
Key
words:
Cassia senna, genotypes, Sudan, genetic diversity,
RAPD. |
