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Full Length Research Paper
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A MspI PCR-RFLP within
bovin growth hormone gene and its association with sperm
quality traits in Iranian Holstein bulls
A. Gorbani1*, R. Vaez Torshizi2, M.
Bonyadi3,4 and C. Amirinia5
1Department
of Animal Science, Islamic Azad University, Science and
Research Branch, Tehran, Iran.
2Department
of Animal science, Faculty of agriculture,
Tarbiat Modares University, Tehran, Iran.
3Center
of Excellence for Biodiversity, Faculty of Natural Sciences,
University of Tabriz, Tabriz, Iran.
4Liver
and Gastrointestinal Disease Research Center, Tabriz
University of Medical Sciences, Tabriz, Iran.
5Department
of Biotechnology, Animal Science research Institute of Iran,
Karaj, Iran.
*Corresponding author. E- mail:
abolfazlgorbani@gmail.com.
Accepted
14 August, 2009 |
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Abstract |
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The present study was aimed to examine the association of
bovine growth hormone gene polymorphism with sperm quality
traits including sperm volume (SV), sperm concentration (SPCO),
total sperm (TS), fresh sperm motility (FSM), total fresh
motile sperm (TFMS), post thaw sperm motility (PTSM), total
post thaw motile sperm (TPTMS), total sperm dose (TSD) and
testis biometry trait as average testis length (ATL),
average testis width (ATW) and scrotum circumference (SC) in
Iranian Holstein bulls. PCR-RFLP method with Msp-I
restriction enzyme was used for genotyping. The frequency of
the MspI+(C) and MspI-(D) alleles are
0.883 and 0.117, respectively. The genotype frequency for
CC, CD and DD were 0.787, 0.191 and 0.022, respectively. The
DD genotype was omitted of analysis. Mixed model analyses of
sperm quality traits considering genotype and environment as
fixed effects and animal as a random effect suggested that
sire was a significant source of variation (P < 0.001) in
all traits. The CC genotype resulted in a significant
increase in SV (p = 0.022), FSM (p < 0.0001), TFMS (p <
0.0001), PTSM (p < 0.0001), TPTMS (p = 0.0067), TSD (p =
0.025) traits greater than CD genotype. However, CD genotype
had significant effect on ATL (p = 0.0223) and ATW (p =
0.0544) traits, but not on SPCO (p = 0.3319), TS (p =
0.3818) and SC (p = 0.3841). These results indicate that new
molecular markers associated with sperm quality traits can
be used in marker-assisted selection in bulls.
Key
words:
Iranian Holstein bull, PCR-RFLP, bGH-Msp-I,
polymorphism, sperm quality trait. |
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