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Insect
chromosomes preparing methods for genetic researches
Ayla Tüzün*
and Serdar Yüksel
Ankara University Faculty of Science Department of Biology
Tandogan Ankara Turkey.
*Corresponding author. E-mail:
Ayla.Tuzun@science.a-nkara.edu.tr.
Accepted 21 November, 2008 |
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Cytogenetics are almost always based on the examination of the
fixed mitotic chromosomes during the analyses of metaphase.
During this phase of the cycle of cells, the DNA is folded
up and chromatin is strongly condensed. The relative
position of the centromere is constant, which means that the
ratio of the lengths of the two arms is constant for each
chromosome. Importantly, each chromosome displays a unique
banding pattern. Different staining methods showed the
banding pattern (g band), centromere position (c band),
cytophotometric estimation and specific DNA regions (FISH).
This study aims to show the important chromosome staining
methods.
Key words:
Fluorescence in situ hybridization (FISH), chromosome
staining, g- banding, c – banding, cytophotometric
estimation. |
