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Studies on isolation and
partial purification of lysozyme from egg white of the
lovebird (Agapornis species)
R. Saravanan1*, A. Shanmugam1,
Preethi Ashok2, D. Sathish Kumar2, K.
Anand3, Amitesh Suman4 and Fredrick
Robin Devadoss5
1Centre
of Advanced Study in Marine Biology, Annamalai University,
Parangipettai-608 502, Tamil Nadu, India.
2Department
of Biotechnology, SRM Arts & Science College,
Kattankulathur-603 203, Tamil Nadu, India.
3Loyola
College, Chennai-600 034, Tamil Nadu, India.
4Department
of Biotechnology, Amity Institute of Biotechnology, Noida.
5Department
of Physics, The American College, Madurai – 625002, India.
*Corresponding author.
E-mail:
saran_prp@yahoo.com.
Accepted
12 June, 2007 |
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Lysozyme was extracted from the egg white of the eggs of
lovebird (Agapornis sp.) and partially purified using
the dialysis membrane. The amount of lysozyme extracted was
determined through absorbance assays using Micrococcus
lysodeikticus cell walls as substrate. The total protein
content in the crude (0.5 mg/ml) and partially purified
(0.01 mg/ml) enzyme extract was estimated. The presence of
lysozyme enzyme in the egg white was confirmed and its
molecular weight (14.3 kDa) was determined through SDS-PAGE.
Key
words:
Lysozyme,
Agapornis, Micrococcus lysodeikticus,
SDS-PAGE. |
