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Genotoxic effects of
industrial wastewater on Allium cepa L.
Levent Şık1*,
Okan Acar2 and Cüneyt Akı2
1Department of Biology, Science and Art Faculty, Celal Bayar
University, Muradiye-Manisa/Turkey.
2Department
of Biology, Science and Art Faculty, Çanakkale Onsekiz Mart
University, Çanakkale/Turkey.
*Corresponding author. E-mail:
levents@bayar.edu.tr.
Tel.: +902362412151.
Accepted
16 March, 2009 |
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The aim of this research is to study the effects of different
concentrations of water on
both incoming and outgoing in central biological and
chemical wastewater treatment plant in Manisa (Turkey)
organized industrial zone (MOIZ) on the Allium cepa
L. root meristems, having been rooted in distilled water for
48 h. The union bulbs were kept in the 100% concentrations
of the refined water (RW) and of 10, 25, 50 and 100%
concentrations of unrefined water (UW). Distilled water was
used for the control samples. It was determined that
wastewater reduced the rate of the mitotic division of
different concentrations and increased the mitotic
anomalies. Mitotic index was found to be 33.8, 31.2, 23.6
and 16.7% in the control group, RW, 10% concentration of the
UW, and 25% concentration of the UW, respectively. On the
other hand, the rates of Mitosis / (Anaphase + Telophase)
were 0.23, 0.28, 0.42, 0.71 in the control group, RW, %
concentration of the UW, and 25% concentration of the UW,
respectively. Plant growth was interrupted in the 50 and
100% concentrations of the UW and the mitotic division was
inhibited. No anomalies were encountered in the control
group. In the RW, a low rate of anomaly was observed, while
in the different concentrations of the UW, chromosomal
aberrations such as high frequency of lagging chromosome,
irregular distribution, polar slips, horizontal division and
sticky chromosome were observed.
Key
words:
Wastewater, refined water, mitotic index, chromosomal
aberrations. |
