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Protein landmarks for
diversity assessment in wheat genotypes
M. F. Siddiqui*
and N. Naz
Federal Seed Certification and Registration Department (FSC
and RD), Islamabad, Pakistan.
*Corresponding author. E-mail:
Send2biotech@yahoo.com.
Tel: +923339017644.
Accepted 6 March, 2009 |
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Grain proteins from 10
Pakistani registered wheat genotypes were
evaluated for diversity assessment based on sodium
dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE).
Genetic diversity was evaluated via UPGMA cluster analysis
by constructing dendrogram of fractions of proteins, which
were used for the calculation of similarity coefficients
between these varieties. The greatest similarity (95%) was
observed between Shahkar-95 and Mehran-89, while the lowest
similarity (16%) was observed between Parwaz-94 and
Abadgar-93.
Adoption of this technology would be useful to plant
protection regulatory systems, especially for plant variety
identification and registration of new plant varieties,
breeding programs and protection purposes.
Key words:
SDS-PAGE, genetic diversity, coefficient of similarity. |
