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Full Length Research Paper
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Regeneration
of begonia plantlets by direct organogenesis
Y. Y. Mendi1*,
P. Curuk1, E. Kocaman1, C.Unek1,
S. Eldogan1, G. Gencel1 and S. Cetiner2
1Laboratory
of Biotechnology, Department of Horticulture, Faculty of
Agriculture, University of Cukurova, Adana, Turkey.
2Faculty
of Engineering and Natural Sciences, University of Sabanci,
Tuzla, Istanbul, Turkey.
*Corresponding author. E-mail:
yesimcan@cu.edu.tr.
Fax: 00903223386615.
Abbreviations: BA, 6-Benzyladenine; IAA,
indole-3-aceticacid; NAA, 1-naphthyleneacetic acid.
Accepted 19 February, 2009 |
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Abstract |
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The economic importance of ornamentals worldwide suggests a
bright future for ornamental breeding. Rapid progress in
plant molecular biology has great potentials to contribute
to the breeding of novel ornamental plants utilizing
recombinant DNA technology. The plant cell, tissue or organ
culture of many ornamental species and their regeneration
are essential for providing the material and systems for
their genetic manipulation, and this is therefore the first
requirement of genetic engineering. In this research,
different concentration of BA (0.0, 0.5, 1.0, 2.0 mgl-¹
with NAA (0.0, 0.5, 1.0 mgl-¹) and BA (0.0, 0.5,
1.0, 2.0 mgl-¹) with IAA (0.0, 0.5, 1.0, mgl-¹)
were investigated to optimize regeneration of Begonia
elatior cv. Toran orange. The best regeneration and growth were obtained from the media containing
2.0 mgl-¹ BA and 1.0 mgl-¹ NAA (70%)
followed by 1.0 mgl-¹ BA and 0.5 mgl-¹
NAA (50%), 1.0 mgl-¹ BA and 1.0 mgl-¹
NAA (20%) in BA - NAA combination. The media with BA - IAA
combination showed that the best regeneration was 0.5 mgl-¹
BA and 0.5 mgl-¹ IAA (43%) followed by 0.5 mgl-¹
BA and 1.0 mgl-¹ IAA (23%).
Key
words:
Begonia,
Begonia tuberus,
ornamental, regeneration, direct organogenesis. |
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