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Using current molecular
techniques for rapid differentiation of Salmonella
Typhi and Salmonella Typhimurium
R. Jordan, E. van Heerden,
C. J. Hugo and L. A. Piater*
Department
of Microbial, Biochemical and Food Biotechnology, University
of the Free State, Bloemfontein, South Africa.
*Corresponding author. E-mail:
piaterla.sci@ufs.ac.za.
Tel.: +27 51 401 2714. Fax: +27 51 444 3219.
Accepted
2 February, 2009 |
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Typhoid fever is responsible for the deaths of many people
annually. However, conventional and time-consuming detection
methods for Salmonella Typhi still dominate. By using
a molecular based approach, it was possible to identify
Salmonella Typhi by amplifying two specific genes (viaB
and tyv) and by using RFLP analysis on the 16S rRNA
gene as a first step for identification. We were also able
to identify Salmonella Typhi using multiple gene
targets in a single multiplex PCR reaction. Here we show
that, as opposed to conventional methods, molecular based
approaches are more rapid and should thus be used for any
initial detection of Salmonella Typhi.
Key
words:
Detection, API, BIOLOG, RFLP, PCR, 16S rRNA Salmonella
Typhi, Salmonella Typhimurium. |
