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Responses of mRNA
expression of PepT1 in small intestine to graded duodenal
soybean small peptides infusion in lactating goats
Liu Hui1,2,
Wang Ling1,2,3, Cao Zhi-jun1,2, Li
Sheng-li1,2* and Wang Li-bing1,2*
1College
of Animal Science and Technology, China Agricultural
University, Beijing 100193, P. R. China.
2National
Key Laboratory of Animal Nutrition, Beijing, 100193, China.
3College
of Agriculture, Ningxia University, Ningxia, 750021, China.
*Corresponding author. E-mail:
lisheng0677@vip.sina.com. Tel:/Fax: +86-10-6273-1254.
Accepted
20 April, 2009 |
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To study the effect of circulation small peptides
concentration on mRNA expression in small intestine, graded
amount of soybean small peptides (SSP) were infused into
lactating goats through duodenal fistulas. Peptide-bound
amino acid (PBAA) concentration in arterial plasma and the
mRNA expression of PepT1 was detected in the current study.
The results showed
that concentrations of all peptide-bound amino acids (PBAA)
increased and
the activity of PepT1 in duodenum tissue was enhanced by SSP
infusion. The PepT1expression in duodenum tissue was
significantly increased with
the increment of amounts of SSP infusion (P<0.05). That
in ileum tissue was increased with
the increment of the amounts of SSP infusion; the increment
of
Pep T1
expression was significant only in the 180 g/d treatment
(P<0.05).
mRNA
abundance of PepT1 in jejunum tissue was
1.1 to
1.8 to
2.4 times
in SSP infusion group
as compared with the control group. In the 120 and 180 g/d
treatment, the difference of
PepT1
expression in jejunum was significant (P<0.05). The data
suggested
Pep T1 expression in duodenum, ileum and jejunum tissue was
enhanced by an increase in circulation small peptide
concentration, and the duodenum might be a main position of
small peptides absorption in intestine.
Key words:
PepT1, mRNA expression, soybean small peptides, small
intestine, lactating goats. |
